Regulatory
PsaDintron

Part:BBa_K2984046

Designed by: Darius Rauch   Group: iGEM19_Humboldt_Berlin   (2019-10-16)


PsadIntron - Promoter for enhanced expression - A1-A3

This part was designed with the AR promoter as a model. The AR Promoter is composed of the HSP70 promoter fused to the RbcS2 intron. We followed a similar approach by fusing the RbcS2 intron to the end of Psad promoter , hoping to achieve a higher expression yield. Every CDS assembled with this part is then fused to the RbcS2 intron. This part is a part of the Chlamy-HUB-Collection.It is desgined according to MoClo standard carrying A1-A3 overhangs.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 19
    Illegal XhoI site found at 997
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None